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KMID : 1007519980070030172
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Food Science and Biotechnology
1998 Volume.7 No. 3 p.172 ~ p.176
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Isolation and Characterization of Lactic Acid Bacteria Producing Bacteriocin Active against Listeria monocytogenes with Microtiter Plate Method
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Kang Dong-Hyun
Daniel Y.C. Fung
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Abstract
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Lactic acid bacteria (LAB) producing bacteriocin active against Listeria monocytogenes were isolated from food samples with microtiter plate method. Each well of microtiter plate contained 25 μL of screened crude bacteriocin and 75 μL of Modified Oxford (MOX, Oxoid) medium inoculated with L. monocytogenes (ca. 5.0 log CFU/mL). L. monocytogenes produced black color on MOX medium. Inhibition of L. monocytogenes by crude bacteriocin can be easily determined using the color change of the medium in microtiter plate well. Among the 312 strains of microorganisms isolated from food, screened 25 strains of LAB produced bacteriocin active against L. monocytogenes. All 25 screened microorganisms were catalase negative and which was identified as Lactobacillus sp. KF-171 produced the strongest bacteriocin active against L. monocytogenes. Bacteriocin produced from Lactobacillus sp. KF-171 were collected with pH change method and evaluated its characteristics. Bacteriocin from Lactobacillus sp. KF-171 was stable between at pH 2.0-6.0 and was heat stable proteinaceous material. The bacteriocin was not active against gram-negative bacteria. When Lactobacillus sp. KF-171 was incubated with L. monocytogenes in sterilized skim milk, the LAB dramatically controlled the number of L. monocytogenes at 37℃ for 16 hr.
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